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mouse anti-muc 5ac  (rdi research diagnostics)

 
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    rdi research diagnostics mouse anti-muc 5ac
    Mouse Anti Muc 5ac, supplied by rdi research diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti-muc 5ac/product/rdi research diagnostics
    Average 90 stars, based on 1 article reviews
    mouse anti-muc 5ac - by Bioz Stars, 2026-03
    90/100 stars

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    Image Search Results


    Left lung cancer. (a) Gross view shows an ill-defined gelatinous tumor with a diffuse pneumonia-like consolidation. Bar, 1 cm. (b) Columnar tumor cells with intracytoplasmic vacuoles reveal a papillary growth pattern. H&E stain, ×200. (c) Multiple mucin-producing tumor cells. Alcian blue stain, ×200. (d) Several tumor cells are positive for MUC 5AC. MUC 5AC immunostain, ×200.

    Journal: Case Reports in Oncological Medicine

    Article Title: Annexin A2 Expression in Aerogenous Metastasis of Pulmonary Invasive Mucinous Adenocarcinoma: A Case Report including Immunohistochemical Analysis

    doi: 10.1155/2019/5064852

    Figure Lengend Snippet: Left lung cancer. (a) Gross view shows an ill-defined gelatinous tumor with a diffuse pneumonia-like consolidation. Bar, 1 cm. (b) Columnar tumor cells with intracytoplasmic vacuoles reveal a papillary growth pattern. H&E stain, ×200. (c) Multiple mucin-producing tumor cells. Alcian blue stain, ×200. (d) Several tumor cells are positive for MUC 5AC. MUC 5AC immunostain, ×200.

    Article Snippet: Mouse anti-human ANX A2 antibody (clone 5/Annexin II, BD Transduction Laboratories, USA) [ ], mouse anti-human MUC 5AC antibody (clone 45M1, Thermo Fisher Scientific, USA), and mouse anti-human TTF-1 antibody (clone SP141, Biocare Medical, USA) were used as the primary antibodies.

    Techniques: Staining

    Overexpression of FIBCD1 modulates mucin protein production. A549 sham- and FIBCD1-transfected cells were seeded at a density of one million cells in 2 mL of media per well of a 6-well tissue culture plate and serum-starved over night prior to incubation with DPBS or 500 μg/mL AIF in 2 mL serum-free media for 0, 4, 8, and 24 h. (A) Western blot analysis was performed as described using 19 μg of cell lysate per well. Lane 1, 3, 5, 7, 9, 11, 13: A549 sham-transfected cells, Lane 2, 4, 6, 8, 10, 12, 14: A549 FIBCD1-transfected cells. The Western blot shown is from one of three independent experiments yielding similar results. Color is adjusted to gray scale. (B–D) Western blots were quantified using ImageJ software. The values obtained after 8 h are expressed as mean ± SEM of the ratio of MUC-1 (B) , MUC-13 (C) , and MUC-5AC (D) to GAPDH from three independent experiments. Data were analyzed by two-way ANOVA, following Tukey's test, # p < 0.05, ## p < 0.01, and ### p < 0.001 relative to control cells. * p < 0.05, ** p < 0.01, and *** p < 0.001 relative to A549 sham cells stimulated with the same stimulant.

    Journal: Frontiers in Immunology

    Article Title: FIBCD1 Binds Aspergillus fumigatus and Regulates Lung Epithelial Response to Cell Wall Components

    doi: 10.3389/fimmu.2018.01967

    Figure Lengend Snippet: Overexpression of FIBCD1 modulates mucin protein production. A549 sham- and FIBCD1-transfected cells were seeded at a density of one million cells in 2 mL of media per well of a 6-well tissue culture plate and serum-starved over night prior to incubation with DPBS or 500 μg/mL AIF in 2 mL serum-free media for 0, 4, 8, and 24 h. (A) Western blot analysis was performed as described using 19 μg of cell lysate per well. Lane 1, 3, 5, 7, 9, 11, 13: A549 sham-transfected cells, Lane 2, 4, 6, 8, 10, 12, 14: A549 FIBCD1-transfected cells. The Western blot shown is from one of three independent experiments yielding similar results. Color is adjusted to gray scale. (B–D) Western blots were quantified using ImageJ software. The values obtained after 8 h are expressed as mean ± SEM of the ratio of MUC-1 (B) , MUC-13 (C) , and MUC-5AC (D) to GAPDH from three independent experiments. Data were analyzed by two-way ANOVA, following Tukey's test, # p < 0.05, ## p < 0.01, and ### p < 0.001 relative to control cells. * p < 0.05, ** p < 0.01, and *** p < 0.001 relative to A549 sham cells stimulated with the same stimulant.

    Article Snippet: The anti-mucin (MUC) antibodies rabbit monoclonal anti-MUC-1 antibody (#ab45167), rabbit polyclonal anti-MUC-13 antibody (#ab65109), and mouse monoclonal anti-MUC-5AC antibody (#ab11335) were ordered from Abcam plc, Cambridge, United Kingdom.

    Techniques: Over Expression, Transfection, Incubation, Western Blot, Software